Development of an Indirect ELISA with Artificially Synthesized N Protein of PPR Virus
نویسندگان
چکیده
منابع مشابه
Development of an indirect ELISA with artificially synthesized N protein of PPR virus.
The full-length gene encoding the nucleocapsid (N) protein of the virus (PPRV) responsible for an outbreak of peste des petits ruminants in Tibet in 2007 was synthesized in two stages using overlapping PCR without the need for viral genomic cDNA as template. The full-length N gene was successfully expressed in Escherichia coli, and the purified gene product bound to monoclonal antibody raised a...
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ژورنال
عنوان ژورنال: Intervirology
سال: 2012
ISSN: 1423-0100,0300-5526
DOI: 10.1159/000322220